[1]温鑫,董艳辉,秦永军.藜麦功能基因的研究进展[J].江苏农业科学,2025,53(19):1-7.
　Wen Xin,et al.Research progress on functional gene in quinoa[J].Jiangsu Agricultural Sciences,2025,53(19):1-7.
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藜麦功能基因的研究进展()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第19期
页码:
1-7
栏目:
专论与综述
出版日期:
2025-10-05

文章信息/Info

Title:
Research progress on functional gene in quinoa
作者:
温鑫1; 董艳辉2; 秦永军2
1.山西农业大学山西有机旱作农业研究院/有机旱作农业山西省重点实验室，山西太原 030031； 2.山西农业大学生命科学学院，山西太原 030031
Author(s):
Wen Xin; et al
关键词:
藜麦; 基因组学; 逆境响应; 发育调控; 营养代谢; 功能验证; 改良育种
Keywords:
-
分类号:
S188
DOI:
-
文献标志码:
A
摘要:
藜麦是一种源于南美洲安第斯山区的伪谷物作物，因具有优异的耐盐、耐旱和耐寒特性，且富含多种氨基酸、高蛋白低脂等，成为保障全球粮食安全和发展生态农业的潜在重要作物。自2008年山西省忻州市静乐县引种藜麦以来，现已培育出多个地方品种，以适应我国北方地区种植和推广。随着藜麦高质量基因组(2017年)和染色体组装(2023年)的完成，藜麦分子水平的研究真正开启。本文从逆境响应、营养代谢与发育调控等方面对藜麦功能基因进行综述，重点分析抗旱、耐盐、耐寒和耐热等非生物胁迫相关基因的发掘，同时总结过表达和基因沉默载体的病毒递送系统在基因验证方面的研究，最后探讨基因编辑技术在当前藜麦研究中应用的卡脖子问题以及未来的研究方向。
Abstract:
-

参考文献/References:

［1］ Bazile D，Jacobsen S E，Verniau A. The global expansion of quinoa：trends and limits［J］. Frontiers in Plant Science，2016，7：622.
［2］王鑫. 藜麦在山西不同生态区域的适应性研究［D］. 太谷：山西农业大学，2018.
［3］杨钊，黄杰，魏玉明，等. 不同地区主栽藜麦品种品质分析与评价［J］. 寒旱农业科学，2024(7)：605-610.
［4］侯丽媛，陈禺怀，王育川，等. 干旱胁迫时间对藜麦苗期生理特性的影响及耐旱性评价［J］. 核农学报，2024，38(11)：2237-2246.
［5］董艳辉，于宇凤，温鑫，等. 基于高通量测序的藜麦连作根际土壤微生物多样性研究［J］. 华北农学报，2019，34(2)：205-211.
［6］Jarvis D E，Ho Y S，Lightfoot D J，et al. The genome of Chenopodium quinoa［J］. Nature，2017，542(7641)：307-312.
［7］Rey E，Maughan P J，Maumus F，et al. A chromosome-scale assembly of the quinoa genome provides insights into the structure and dynamics of its subgenomes［J］. Communications Biology，2023，6(1)：1263.
［8］陈薇薇，冯鹏睿，张永清，等. 藜麦研究态势与发展趋势分析［J］. 中国农学通报，2024，40(22)：8-16.
［9］张述伟，宗营杰，黄琳丽，等. 近十年国内外藜麦研究进展与热点分析［J］. 中国农学通报，2024，40(5)：145-152.
［10］李玲红，苟彤，任爱霞，等. 藜麦基因组学与重要农艺性状位点研究进展［J］. 遗传，2022，44(11)：1009-1027.
［11］董艳辉，王育川，温鑫，等. 藜麦育种技术研究进展［J］. 中国种业，2020(1)：8-13.
［12］侯丽媛，董艳辉，李亚莉，等. 藜麦抗旱性研究进展与展望［J］. 江苏农业科学，2021，49(11)：22-28.
［13］Hinojosa L，González J A，Barrios-Masias F H，et al. Quinoa abiotic stress responses：a review［J］. Plants，2018，7(4)：106.
［14］Li H X，Jiang C H，Liu J N，et al. Genome-wide identification of the AAT gene family in quinoa and analysis of its expression pattern under abiotic stresses［J］. BMC Genomics，2025，26(1)：298.
［15］Qian G T，Yang J R，Wang M Y，et al. Identification of the Dof gene family in quinoa and its potential role in regulating flavonoid synthesis under different stress conditions［J］. Biology，2025，14(4)：446.
［16］侯丽媛，贾举庆，姜晓东，等. 藜麦WRKY基因的进化与多胁迫条件下的转录应答［J］. 草业学报，2022，31(9)：168-182.
［17］Iqbal H，Chen Y N，Waqas M，et al. Differential response of quinoa genotypes to drought and foliage-applied H2O2 in relation to oxidative damage，osmotic adjustment and antioxidant capacity［J］. Ecotoxicology and Environmental Safety，2018，164：344-354.
［18］Askar A，Gul H，Rauf M，et al. Drought stress alleviation in Chenopodium quinoa through synergistic effect of silicon and molybdenum via triggering of SNF1-associated Protein kinase 2 signaling mechanism［J］. Phyton，2024，93(9)：2455-2478.
［19］Zhu X L，Liu W Y，Wang B Q，et al. Molecular and physiological responses of two quinoa genotypes to drought stress［J］. Frontiers in Genetics，2024，15：1439046.
［20］Bakhtari B，Razi H，Alemzadeh A，et al. Identification and characterization of the Quinoa AP2/ERF gene family and their expression patterns in response to salt stress［J］. Scientific Reports，2024，14(1)：29529.
［21］Ding P C，Tang P，Li X F，et al. Genome-wide identification，phylogeny and expression analysis of the R2R3-MYB gene family in quinoa (Chenopodium quinoa) under abiotic stress［J］. Functional Plant Biology，2024，51：FP23261.
［22］Xue G X，Fan Y，Zheng C Y，et al. bHLH transcription factor family identification，phylogeny，and its response to abiotic stress in Chenopodium quinoa［J］. Frontiers in Plant Science，2023，14：1171518.
［23］Zhang D F，Zhu X L，Du X F，et al. Identification of the Valine-Glutamine gene family in Chenopodium quinoa Willd and analysis of its expression pattern and subcellular localization under drought stress［J］. BMC Genomics，2025，26(1)：252.
［24］Sun W J，Wei J L，Wu G M，et al. CqZF-HD14 enhances drought tolerance in quinoa seedlings through interaction with CqHIPP34 and CqNAC79［J］. Plant Science，2022，323：111406.
［25］Zou C S，Chen A J，Xiao L H，et al. A high-quality genome assembly of quinoa provides insights into the molecular basis of salt bladder-based salinity tolerance and the exceptional nutritional value［J］. Cell Research，2017，27(11)：1327-1340.
［26］许浩宇，赵颖，阮倩，等. 不同混合盐碱下藜麦幼苗的抗性研究［J］. 草业学报，2023，32(1)：122-130.
［27］Bhm J，Messerer M，Müller H M，et al. Understanding the molecular basis of salt sequestration in epidermal bladder cells of Chenopodium quinoa［J］. Current Biology，2018，28(19)：3075-3085.
［28］Food and Agriculture Organization. Temperature change statistics 1961—2024-Global，regional and country trends［R］. Roma：FAOSTAT Analytical Briefs，2025，101：2-6.
［29］Tovar J C，Quillatupa C，Callen S T，et al. Heating quinoa shoots results in yield loss by inhibiting fruit production and delaying maturity［J］. The Plant Journal，2020，102(5)：1058-1073.
［30］Alvar-Beltrán J，Verdi L，Dalla Marta A，et al. The effect of heat stress on quinoa (cv. titicaca) under controlled climatic conditions［J］. The Journal of Agricultural Science，2020，158(4)：255-261.
［31］Tashi G B，Zhan H S，Xing G W，et al. Genome-wide identification and expression analysis of heat shock transcription factor family in Chenopodium quinoa Willd［J］. Agronomy，2018，8(7)：103.
［32］Xie H，Zhang P，Jiang C H，et al. Combined transcriptomic and metabolomic analyses of high temperature stress response of quinoa seedlings［J］. BMC Plant Biology，2023，23(1)：292.
［33］Fu R，Liang X Y，Li J J，et al. Comparative transcriptomic analyses reveal key pathways in response to cold stress at the germination stage of quinoa (Chenopodium quinoa Willd.) seeds［J］. Plants，2025，14(8)：1212.
［34］Zhang L Y，Jiang G F，Wang X Q，et al. Identifying core genes related to low-temperature stress resistance in quinoa seedlings based on WGCNA［J］. International Journal of Molecular Sciences，2024，25(13)：6885.
［35］Zhang L Y，Xia Y B，Jiang G F，et al. Identification of quinoa B3 gene family and its expression pattern in response to low temperature stress［J］. Genetic Resources and Crop Evolution，2025，72(4)：4345-4360.
［36］Fu D Y，Song Y，Wu S F，et al. Regulation of alternative splicing by CBF-mediated protein condensation in plant response to cold stress［J］. Nature Plants，2025，11(3)：505-517.
［37］Zheng L，Zhao Y W，Gan Y F，et al. Full-length transcriptome sequencing reveals the impact of cold stress on alternative splicing in quinoa［J］. International Journal of Molecular Sciences，2022，23(10)：5724.
［38］Guo Y R，Wang Q C，Xie H，et al. Analysis of root response mechanism of quinoa seedlings to waterlogging stress based on metabolome［J］. Journal of Plant Growth Regulation，2024，43(7)：2251-2264.
［39］Wang X Q，Zong X M，Jiang G F，et al. Genome-wide identification and expression analysis of C2H2 zinc finger protein transcription factor in quinoa［J］. Genetic Resources and Crop Evolution，2025，72(7)：7779-7794.
［40］Zhang S，Liu J，Shi L，et al. Identification of core genes associated with different phosphorus levels in quinoa seedlings by weighted gene co-expression network analysis［J］. BMC Genomics，2023，24(1)：399.
［41］Wang X X，Wu H，Manzoor N，et al. The identification of AMT family genes and their expression，function，and regulation in Chenopodium quinoa［J］. Plants，2024，13(24)：3524.
［42］马玉馨，潘雪宇，张钰泉，等. 藜麦皂苷生物合成的转录组学分析［J/OL］. 分子植物育种，2024：1-18(2024-03-26)［2025-03-01］. https://www.cnki.com.cn/Article/CJFDTotal-FZZW20240318008.htm.
［43］Trinh M D L，Visintainer D，Günther J，et al. Site-directed genotype screening for elimination of antinutritional saponins in quinoa seeds identifies TSARL1 as a master controller of saponin biosynthesis selectively in seeds［J］. Plant Biotechnology Journal，2024，22(8)：2216-2234.
［44］Yang J D，Wang Y Y，Sun J Y，et al. Metabolome and transcriptome association analysis reveals mechanism of synthesis of nutrient composition in quinoa (Chenopodium quinoa Willd.) seeds［J］. Foods，2024，13(9)：1325.
［45］Manzoor N，Yuan J H，Dongcheng W H，et al. Integrated transcriptomic and proteomic analyses revealed molecular mechanisms underlying nutritional changes during seed development of Chenopodium quinoa［J］. Genomics，2025，117(3)：111045.
［46］Wang Q C，Shi J R，Liu J N，et al. Integration of transcriptome and metabolome reveals the accumulation of related metabolites and gene regulation networks during quinoa seed development［J］. Plant Molecular Biology，2024，114(1)：10.
［47］时小东，孙梦涵，吴琪，等. 基于藜麦转录组的脂肪酸生物合成途径解析［J］. 广西植物，2020，40(12)：1721-1731.
［48］王春妹，王梅，王红霞，等. 干旱胁迫下藜麦种子糖代谢转录组学研究［J］. 植物遗传资源学报，2024，25(8)：1370-1384.
［49］丰扬，郭凤根，王仕玉，等. 藜麦Cq6GT基因的克隆与表达分析［J］. 植物生理学报，2022，58(10)：2017-2024.
［50］Matías J，Cruz V，Reguera M. Heat stress impact on yield and composition of quinoa straw under Mediterranean field conditions［J］. Plants，2021，10(5)：955.
［51］Maldonado-Taipe N，Barbier F，Schmid K，et al. High-density mapping of quantitative trait loci controlling agronomically important traits in quinoa (Chenopodium quinoa Willd.)［J］. Frontiers in Plant Science，2022，13：916067.
［52］王春妹，张敏，张利苹，等. 藜麦(Chenopodium quinoa)重要农艺性状SNP标记发掘［J/OL］. 分子植物育种，2023：1-23［2025-03-01］. https://link.cnki.net/urlid/46.1068.S.20231227.1330.012.
［53］Rahman H，Vikram P，Hu Y L，et al. Mining genomic regions associated with agronomic and biochemical traits in quinoa through GWAS［J］. Scientific Reports，2024，14(1)：9205.
［54］Wu Q，Bai X，Zhao W，et al. Investigation into the underlying regulatory mechanisms shaping inflorescence architecture in Chenopodium quinoa［J］. BMC Genomics，2019，20(1)：658.
［55］林彤，袁程，董陈文华，等. 藜麦配子发育相关基因CqSTK的筛选及功能分析［J］. 生物技术通报，2024，40(8)：83-94.
［56］孙慧琼，张春来，王锡亮，等. 藜麦FLS基因家族的鉴定、表达及DNA变异分析［J］. 生物技术通报，2024，40(7)：172-182.
［57］徐宏申，姜晓东，王锡亮，等. 藜麦生长素结合蛋白基因ABP1的鉴定与表达分析［J］. 植物生理学报，2024，60(7)：1157-1167.
［58］Golicz A A，Steinfort U，Arya H，et al. Analysis of the quinoa genome reveals conservation and divergence of the flowering pathways［J］. Functional & Integrative Genomics，2020，20(2)：245-258.
［59］张敏，王梅，王红霞，等. 藜麦叶花青素生物合成转录组-代谢组联合分析［J］. 河北农业大学学报，2024，47(1)：37-48.
［60］王慧，曹天光，秦垒，等. 碳离子辐射藜麦多酚含量变化的多组学分析［J/OL］. 分子植物育种，2023：1-20(2023-07-25)［2025-03-01］. https：//kns.cnki.net/kcms/detail/46.1068.S.20230724.2116.011.html.
［61］Han H N，Qu Y S，Wang Y C，et al. Transcriptome and small RNA sequencing reveals the basis of response to salinity，alkalinity and hypertonia in quinoa (Chenopodium quinoa Willd.)［J］. International Journal of Molecular Sciences，2023，24(14)：11789.
［62］Zhao H M，Cao H Q，Zhang M，et al. Genome-wide identification and characterization of SPL family genes in Chenopodium quinoa［J］. Genes，2022，13(8)：1455.
［63］Ogata T，Toyoshima M，Yamamizo-Oda C，et al. Virus-mediated transient expression techniques enable functional genomics studies and modulations of betalain biosynthesis and plant height in quinoa［J］. Frontiers in Plant Science，2021，12：643499.
［64］Melgar A E，Palacios M B，Tosar L J M，et al. A novel and efficient Apple latent spherical virus-based gene silencing method for functional genomic studies in Chenopodium quinoa［J］. Scientia Horticulturae，2024，333：113258.
［65］Xiao X L，Meng F X，Satheesh V，et al. An Agrobacterium-mediated transient expression method contributes to functional analysis of a transcription factor and potential application of gene editing in Chenopodium quinoa［J］. Plant Cell Reports，2022，41(10)：1975-1985.
［66］Hesami M，Daneshvar M H. Development of a regeneration protocol through indirect organogenesis in Chenopodium quinoa Willd［J］. Indo-American Journal of Agricultural and Veterinary Sciences，2016，4(1)：25-32.
［67］Regalado J J，Tossi V E，Burrieza H P，et al. Micropropagation protocol for coastal quinoa［J］. Plant Cell，Tissue and Organ Culture，2020，142(1)：213-219.
［68］Hesami M，Naderi R，Yoosefzadeh-Najafabadi M. Optimizing sterilization conditions and growth regulator effects on in vitro shoot regeneration through direct organogenesis in Chenopodium quinoa［J］. BioTechnologia，2018，99(1)：49-57.
［69］Gong Y，Guo S L，Wu X L，et al. Direct organogenesis protocol for in vitro propagation of Chenopodium quinoa［J/OL］. Research Square，2022(2022-09-12)［2025-03-01］.https：//doi.org/10.21203/rs.3.rs-1935859/v1.
［70］王宇. 藜麦不同U6启动子的克隆与功能分析及靶向CqASMT12基因CRISPR/Cas9编辑载体的构建［D］. 烟台：烟台大学，2020.
［71］高爱红，张侠，曹萌，等. 藜麦ANT基因家族的鉴定及其在愈伤组织中的表达分析［J］. 山东农业科学，2025，57(4)：22-31.
［72］Sidorov V，Maughan P，Yang P Z. The development of an in vitro floral culture transformation system for quinoa［J］. In Vitro Cellular & Developmental Biology -Plant，2024，60：742-750.

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[1]陆敏佳,莫秀芳,王勤,等.藜麦基因组DNA提取方法的比较[J].江苏农业科学,2014,42(04):42.
　Lu Minjia,et al.Comparison of extraction methods of genomic DNA from Chenopodium quinoa[J].Jiangsu Agricultural Sciences,2014,42(19):42.
[2]俞涵译,蒋玉蓉,毛泽阳,等.藜麦愈伤组织诱导体系优化研究[J].江苏农业科学,2015,43(03):26.
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备注/Memo

备注/Memo:
收稿日期：2025-05-06
基金项目：山西省基础研究计划(编号：20210302124366)。
作者简介：温鑫(1992—)，女，山西和顺人，硕士，助理研究员，从事作物基因组学与分子育种研究。E-mail：x.wen@sxau.edu.cn。
通信作者：秦永军，博士，研究员，主要从事作物基因组学与分子育种研究。E-mail：y.qin@sxau.edu.cn。
更新日期/Last Update: 2025-10-05