|本期目录/Table of Contents|

[1]王彪,智倩,薛永常.壳聚糖酶基因片段的克隆及序列分析[J].江苏农业科学,2013,41(09):12-14.
　Wang Biao,et al.Cloning and sequence analysis of chitosanase gene fragment[J].Jiangsu Agricultural Sciences,2013,41(09):12-14.
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壳聚糖酶基因片段的克隆及序列分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年09期
页码:
12-14
栏目:
生物技术
出版日期:
2013-09-25

文章信息/Info

Title:
Cloning and sequence analysis of chitosanase gene fragment
作者:
王彪;  智倩;  薛永常
大连工业大学生物工程学院，辽宁大连 116034
Author(s):
Wang Biao; et al
关键词:
壳聚糖酶基因; 克隆; 序列分析; 同源性
Keywords:
-
分类号:
Q785
DOI:
-
文献标志码:
A
摘要:
由GenBank上发布的壳聚糖酶基因编码区序列设计PCR扩增引物，从笔者所在实验室已筛选的1株具有降解壳聚糖功能的芽孢杆菌中克隆得到壳聚糖酶基因片段，将该片段与pMD20-T载体连接后转化至大肠杆菌DH5α中，测序并与NCBI上已发布的芽孢杆菌属壳聚糖酶编码区序列进行比对分析。结果表明，该片段与来自糖苷水解酶46家族中的Bacillus circulans MH-K1和Bacillus ehimensis EAG1的壳聚糖酶编码区序列同源性分别为71.75%和75.93%；与糖苷水解酶8家族中的Bacillus sp.No.7-M的壳聚糖酶编码区序列同源性为87.73%。初步推断该片段为类似壳聚糖酶基因片段。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2013-01-10
作者简介：王彪(1986—)，男，硕士研究生，研究方向为基因工程。E-mail：polony2008@163.com。
通信作者：薛永常，博士，教授，研究方向为分子生物学。E-mail：xueych@dlpu.edu.cn。
更新日期/Last Update: 2013-09-25