|本期目录/Table of Contents|

[1]翟立红,周兰庭,韩鹏,等.玉米Argoanute基因家族的全长cDNA克隆与表达分析[J].江苏农业科学,2016,44(04):41-45.
　Zhai Lihong,et al.Full-length cDNA cloning and expression analysis of Argonaute genes from Zea mays L.[J].Jiangsu Agricultural Sciences,2016,44(04):41-45.
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玉米Argoanute基因家族的全长cDNA克隆与表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年04期
页码:
41-45
栏目:
生物技术
出版日期:
2016-04-25

文章信息/Info

Title:
Full-length cDNA cloning and expression analysis of Argonaute genes from Zea mays L.
作者:
翟立红1; 周兰庭1; 韩鹏1; 腾峰1; 2
1.湖北文理学院生物化学与分子生物学系，湖北襄阳 441053；2.湖北腾龙种业有限公司，湖北利川 445400
Author(s):
Zhai Lihong; et al
关键词:
Argonaute基因; 克隆; 5′非翻译外显子; 表达
Keywords:
-
分类号:
S513.01；Q785
DOI:
-
文献标志码:
A
摘要:
Argonaute(AGO)蛋白是小RNA分子诱导的沉默复合体(RISC)的核心因子，以玉米自交系B73为材料，利用RLM-RACE技术，克隆得到玉米17个AGO基因的全长cDNA序列，发现6个成员具有5′非翻译外显子，可能其表达受此机制调控。同时，利用半定量RT-PCR对其在玉米幼苗不同根系组织(包括初生胚根、冠根、次生胚根和侧根)进行表达分析，结果表明：未检测到ZmAGO5b、ZmAGO18b表达，ZmAGO1a/b、ZmAGO2a、ZmAGO10在各类根系中高水平表达，其他11个ZmAGOs表达水平较低，因此揭示选择性表达不同的ZmAGOs可能是不同类型的根系分化及其功能的精细调控所必需的。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2015-10-29
基金项目：湖北省教育厅科学研究计划中青年人才项目(编号：Q20152601)。
作者简介：翟立红(1983—)，女，河北邢台人，讲师，主要从事玉米遗传育种研究。E-mail：zlh_0302@126.com。
通信作者：腾峰，博士，助理研究员，主要从事玉米育种研究。E-mail：tobenumberone@yeah.net。
更新日期/Last Update: 2016-04-25