|本期目录/Table of Contents|

[1]康俊.紫杉醇关键酶基因TwDBAT在平菇中的遗传转化及表达[J].江苏农业科学,2016,44(10):98-103.
　Kang Jun.Transformation and heterologous expression of TwDBAT from taxol biosynthetic pathway in Pleurotus ostreatus[J].Jiangsu Agricultural Sciences,2016,44(10):98-103.
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紫杉醇关键酶基因TwDBAT在平菇中的
遗传转化及表达(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年10期
页码:
98-103
栏目:
生物技术
出版日期:
2016-10-25

文章信息/Info

Title:
Transformation and heterologous expression of TwDBAT from taxol biosynthetic pathway in Pleurotus ostreatus
作者:
康俊
乌兰察布职业学院，内蒙古乌兰察布 012000
Author(s):
Kang Jun
关键词:
平菇; 紫杉醇; 紫杉醇前体物质; 关键酶基因; TwDBAT基因; 遗传转化; 基因表达
Keywords:
-
分类号:
S188
DOI:
-
文献标志码:
A
摘要:
通过构建含平菇内源gpd启动子、红豆杉中TwDBAT基因的真核表达载体pgFvs-TwDBAT，建立高效的平菇原生质体制备与再生体系；采用PEG介导法，将真核表达载体pgFvs-TwDBAT、pgFvs-hph共转化高温型平菇菌株F7E原生质体，获得可以异源表达TwDBAT基因的平菇菌株。PCR和Southern杂交鉴定结果表明，TwDBAT基因整合进平菇基因组中；经继代培养，TwDBAT基因在转化子中可稳定遗传。RT-PCR结果表明，转化子在mRNA水平上转录了TwDBAT基因。HPLC结果显示，TwDBAT酶可以催化底物10-去乙酰巴卡亭Ⅲ(10-DAB)。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2016-03-31
基金项目：内蒙古自治区高等学校科学研究项目(编号：NJZC14383)。
作者简介：康俊(1982—)，女，内蒙古乌兰察布人，硕士，讲师，主要从事植物病理及遗传育种的教学研究工作。E-mail：kang_jun@163.com。
更新日期/Last Update: 2016-10-25