|本期目录/Table of Contents|

[1]陈满静,任艳,彭秋,等.基于BSA-seq方法定位贵州高粱抗炭疽病害关键遗传区段[J].江苏农业科学,2022,50(5):28-34.
　Chen Manjing,et al.Mapping of key genetic segments of sorghum resistance to anthracnose in Guizhou Province based on BSA-seq method[J].Jiangsu Agricultural Sciences,2022,50(5):28-34.
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基于BSA-seq方法定位贵州高粱抗炭疽病害关键遗传区段(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第5期
页码:
28-34
栏目:
生物技术
出版日期:
2022-03-05

文章信息/Info

Title:
Mapping of key genetic segments of sorghum resistance to anthracnose in Guizhou Province based on BSA-seq method
作者:
陈满静;  任艳;  彭秋;  李青风;  高杰;  邓小锋
贵州省农业科学院旱粮研究所，贵州贵阳 550006
Author(s):
Chen Manjing; et al
关键词:
高粱; 炭疽病; 抗性; 候选区段; BSA-seq
Keywords:
-
分类号:
S435.14
DOI:
-
文献标志码:
A
摘要:
高粱炭疽病是威胁高粱生长的主要病害之一，挖掘高粱抗炭疽病相关基因能够为高粱抗性品种育种打下基础。利用前期田间试验鉴定出的高粱高抗材料F41和高感材料B57进行杂交构建F2:3代分离群体，挑选高梁高抗炭疽病植株和高感炭疽病植株各30株，分别构建2个极端性状的DNA混合池，利用高通量测序技术与集团分离分析法相结合的方法(BSA-seq)进行全基因组重测序和关联分析，定位和抗性性状相关的基因组区段。通过SNP-index及InDel-index方法进行关联分析及对候选区域进行基因注释，共注释到基因143个，其中非同义突变基因49个，移码突变基因16个。研究结果为高粱抗炭疽病分子机制及抗性相关基因的克隆奠定了理论基础。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2021-09-08
基金项目：国家科技支撑计划(编号：2014BAD07B02-2-4)；黔农科院青年基金( 编号：［2018］57号)。
作者简介：陈满静(1991—)，女，贵州三穗人，硕士，助理研究员，主要从事高粱作物栽培技术研究。E-mail：nkycmj@yeah.net。
通信作者：邓小锋，博士，助理研究员，主要从事高粱育种研究。E-mail：dixifor@yeah.net。
更新日期/Last Update: 2022-03-05