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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第45卷

期数:

2017年08期

页码:

91-94

栏目:

植物保护

出版日期:

2017-04-22

文章信息/Info

Title:

Differential effect of reacting temperature on endogenous and exogenous cellulose activities of Coptotermes formosanus Shiraki(Rhinotermitidae)

作者:

曾文慧;  钟俊鸿;  李秋健;  刘炳荣

广东省昆虫研究所/广东省野生动物保护与利用公共实验室/广东省农业害虫综合治理重点实验室，广东广州 510260

Author(s):

Zeng Wenhui; et al

关键词:

台湾乳白蚁; 纤维素酶; 双纤维素水解系统; 反应温度

Keywords:

-

分类号:

S433.89

DOI:

-

文献标志码:

A

摘要:

台湾乳白蚁(Coptotermes formosanus Shiraki)不仅是我国重要的经济害虫，也是研究低等白蚁木质纤维素二元降解系统的模式生物。将台湾乳白蚁消化道解剖分成唾液腺/前肠+中肠(内源)与后肠(外源)2个部分，以台湾乳白蚁生理临界温度区间(15~42 ℃)为反应温度，测定内切葡聚糖酶(endo-1，4-β-D-glucanohydrolasease，简称EG)、纤维二糖水解酶(cellobiohydrases，简称CBH)、β-葡萄糖苷酶 (β-glucosidase，简称BG)以及滤纸酶(filter paper，简称FPA)的活力，旨在探讨反应温度对台湾乳白蚁内源与外源纤维素酶活力的影响差异。结果表明，整头白蚁的FPA与EG酶活力随反应温度变化趋势一致。整头白蚁BG酶在22~42 ℃下反应活力无显著差异。反应温度对外源纤维素酶活力的影响大于对内源纤维素酶活力的影响。后肠EG、BG、CBH及FPA最佳反应温度均为32 ℃，而相应内源纤维素酶最佳反应温度均高于37 ℃。

Abstract:

-

参考文献/References:

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备注/Memo

备注/Memo:

收稿日期：2016-01-15
基金项目：广东省自然科学基金(编号：2015A030313817)。
作者简介：曾文慧(1984—)，女，江西赣州人，硕士，助理研究员，主要从事白蚁生理生化与分子生物学研究。E-mail：zengwenhuigz@hotmail.com。
通信作者：刘炳荣，博士，助理研究员，主要从事白蚁生理生化研究。E-mail：22118509@qq.com。

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更新日期/Last Update: 2017-04-20