|本期目录/Table of Contents|

[1]周苗苗,崔景香.小肽氨基酸组成对体外培养的奶牛乳腺组织乳蛋白合成的影响[J].江苏农业科学,2017,45(18):166-168.
　Zhou Miaomiao,et al.Effects of small peptide amino acid composition on milk protein synthesis in breast tissue in vitro of dairy cows[J].Jiangsu Agricultural Sciences,2017,45(18):166-168.
点击复制

小肽氨基酸组成对体外培养的奶牛
乳腺组织乳蛋白合成的影响(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年18期
页码:
166-168
栏目:
畜牧兽医与水产蚕桑
出版日期:
2017-09-20

文章信息/Info

Title:
Effects of small peptide amino acid composition on milk protein synthesis in breast tissue in vitro of dairy cows
作者:
周苗苗;  崔景香
山东潍坊科技学院动物科学研究所，山东寿光 262700
Author(s):
Zhou Miaomiao; et al
关键词:
小肽; 氨基酸; 赖氨酸二肽; 乳蛋白; 小肽转运载体2; 奶牛乳腺组织; 丰度; αs1酪蛋白基因
Keywords:
-
分类号:
S823.9+11
DOI:
-
文献标志码:
A
摘要:
为研究不同二肽对奶牛乳腺组织中乳蛋白合成的影响，在体外培养的奶牛乳腺组织培养液中分别添加不同的赖氨酸二肽：赖氨酸-赖氨酸(Lys-Lys)、赖氨酸-组氨酸(Lys-His)、赖氨酸-苯丙氨酸(Lys-Phe)、赖氨酸-亮氨酸(Lys-Leu)和赖氨酸-苏氨酸(Lys-Thr)，等量取代培养液中10%游离赖氨酸(赖氨酸总浓度为210 μg/mL)进行培养，试验结束后收集乳腺组织和培养液分别检测基因表达和乳蛋白合成。结果表明，同游离Lys和Lys-Thr、Lys-Lys、Lys-His二肽相比，Lys-Leu和Lys-Phe显著提高了乳腺组织中αs1酪蛋白基因的表达(P<0.05)；5种肽结合Lys均显著提高了小肽转运载体2(PepT2)的mRNA丰度(P<0.05)，Lys-Phe和Lys-Leu比等量的Lys-Thr、Lys-Lys和Lys-His更能促进PepT2基因的表达(P<0.05)。说明小肽不同的氨基酸组成可影响奶牛小肽的摄取和乳蛋白的合成，同亲水性氨基酸组成的小肽相比，疏水性氨基酸组成的小肽更能促进乳腺αs1酪蛋白基因的表达。
Abstract:
-

参考文献/References:

［1］Tagari H，Webb K，Theurer B，et al. Mammary uptake，portal-drained visceral flux，and hepatic metabolism of free and peptide-bound amino acids in cows fed steam-flaked or dry-rolled sorghum grain diets［J］. Journal of Dairy Science，2008，91(2)：679-697.
［2］Mabjeesh S J，Kyle C E，MacRae J C，et al. Vascular sources of amino acids for milk protein synthesis in goats at two stages of lactation［J］. Journal of Dairy Science，2002，85(4)：919-929.
［3］Wang S. Peptide-bound methionine can be a source of methionine for the synthesis of secreted proteins by mammary tissue explants from lactating mice［J］. The Journal of Nutrition，1996，126(6)：1662.
［4］Pan Y，Bender P K，Akers M，et al. Methionine-containing peptides can be used as methionine sources for protein accretion in cultured C2C12 and MAC-T cells［J］. The Journal of Nutrition，1996，126(1)：232.
［5］Wu H H，Yang J Y，Zhao K. Effects of methionine-containing dipeptides on casein αs1 expression in bovine mammary epithelial cells［J］. Journal of Animal Feed Science，2007，16(S2)：7-12.
［6］Zhou M M，Wu Y M，Liu H Y，et al. Role of oligopeptide transporter 2 in bovine mammary gland phenylalanine dipeptide uptake［J］. Chin Anim Nutr，2011，23(8)：1303-1308.
［7］Zhou M M，Wu Y M，Liu H Y，et al. Effects of tripeptide and lactogenic hormones on oligopeptide transporter 2 in bovine mammary gland［J］. J Anim Physiol An N，2011，95(6)：781-789.
［8］Zhou M M，Wu Y M，Liu H Y，et al. Effects of phenylalanine and threonine oligopeptides on milk protein synthesis in cultured bovine mammary epithelial cells［J］. J Anim Physiol An N，2015，99(2)：215-220.
［9］Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding ［J］. Anal Biochem，1976，72(1-2)：248-254.
［10］Daniel H，Kottra G. The proton oligopeptide cotransporter family SLC15 in physiology and pharmacology［J］. Pflügers Archiv，2004，447(5)：610-618.
［11］Matthews D M. Protein absorption［M］. New York：Development and Present State of the Subject，1991.
［12］Takahashi K，Masuda S，Nakamura N，et al. Upregulation of H+-peptide cotransporter PepT2 in rat remnant kidney［J］. American Journal of Physiology (Renal Physiology)，2001，281(6)：1109-1116.

相似文献/References:

[1]王未,毛日文,赵婷,等.玛咖营养成分分析[J].江苏农业科学,2013,41(04):285.
[2]朱伟伟,蓝建京.犀牛角氨基酸组成分析与营养价值评价[J].江苏农业科学,2013,41(04):289.
[3]顾晓云,茆广华,冯伟伟,等.灰树花残渣成分分析[J].江苏农业科学,2014,42(10):303.
　Gu Xiaoyun,et al.Analysis of chemical composition of Grifola frondosa residues[J].Jiangsu Agricultural Sciences,2014,42(18):303.
[4]王豪,徐致远,刘振民,等.不同发酵温度对开菲尔产氨基酸及理化性质的影响[J].江苏农业科学,2014,42(09):236.
　Wang Hao,et al.Effects of different fermentation temperatures on amino acid production and physicochemical properties of kefir[J].Jiangsu Agricultural Sciences,2014,42(18):236.
[5]董占营,何文辉,华雪铭,等.清洁虾氨基酸组成及含量分析[J].江苏农业科学,2013,41(12):318.
　Dong Zhanying,et al.Analysis of components and contents of amino acids in Lysmata amboinensis[J].Jiangsu Agricultural Sciences,2013,41(18):318.
[6]姚正颖,张卫明,孙力军.续随子籽粕饲用概略营养成分测定及氨基酸组成分析[J].江苏农业科学,2013,41(12):321.
　Yao Zhengying,et al.Determination of conventional nutrients and amino acid components of Euphorbia lathyris seed meal[J].Jiangsu Agricultural Sciences,2013,41(18):321.
[7]缪凌鸿,戈贤平,高启平,等.不同体型鳙鱼幼鱼营养成分与品质的比较[J].江苏农业科学,2016,44(04):334.
　Miao Linghong,et al.Composition analysis and nutritional evaluation of different somatotypes of juvenile bighead carp (Aristichthys nobilis)[J].Jiangsu Agricultural Sciences,2016,44(18):334.
[8]盖江涛,沈建凯,王鹏.主要作物中PAL基因家族的鉴定和序列分析[J].江苏农业科学,2016,44(06):45.
　Gai Jiangtao,et al.Identification and sequence analysis of PAL gene family in main crops[J].Jiangsu Agricultural Sciences,2016,44(18):45.
[9]王爱民,窦超,吴文静,等.中华鳖幼鳖肌肉营养成分与品质的评价[J].江苏农业科学,2016,44(01):269.
　Wang Aimin,et al.Evaluation of muscle nutrition composition and quality of juvenile soft-shelled turtle[J].Jiangsu Agricultural Sciences,2016,44(18):269.
[10]白云峰,高立鹏,涂远璐,等.区域性部分非常规饲料的氨基酸组成分析[J].江苏农业科学,2014,42(04):165.
　Bai Yunfeng,et al.Analysis of amino acid composition of regional unconventional feed[J].Jiangsu Agricultural Sciences,2014,42(18):165.

备注/Memo

备注/Memo:
收稿日期：2016-04-29
基金项目：山东省潍坊市科技发展计划(编号：2015GX077)。
作者简介：周苗苗(1983—)，女，山东聊城人，博士，副教授，主要从事反刍动物泌乳生理研究。E-mail：zhoumm0329@163.com。
更新日期/Last Update: 2017-09-20