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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第47卷

期数:

2019年第13期

页码:

222-226

栏目:

贮藏加工与检测分析

出版日期:

2019-07-31

文章信息/Info

Title:

Optimization of liquid fermentation conditions for flavonoids from medicinal fungus Phellinus linteus based on response surface method

作者:

谢春芹¹;  曹正¹;  王春野¹;  姜爱良²;  吴琴燕³;  张雪姣²;  任昂²

1.江苏农林职业技术学院，江苏句容 212400； 2.南京农业大学，江苏南京 210095； 3.镇江市农业科学技术实业公司，江苏句容 212400

Author(s):

Xie Chunqin; et al

关键词:

桑黄; 黄酮; 响应面分析法; 发酵培养基

Keywords:

-

分类号:

S188⁺.4

DOI:

-

文献标志码:

A

摘要:

桑黄是一种珍贵的药用真菌，通过响应面分析法对桑黄的发酵培养基进行优化，进而提高该菌株黄酮类活性物质的产量。利用不同碳源、不同氮源对桑黄进行液体发酵，测定胞内黄酮产量，筛选出最佳种类的碳源和氮源。进一步通过单因素试验考察不同浓度碳、氮源及培养基初始pH值对胞内黄酮产量的影响。利用响应面分析法对桑黄黄酮发酵培养基进行优化，获得黄酮产量最高的培养基组合。结果表明，桑黄产黄酮的最佳碳源是麦芽糖，最佳氮源是酵母提取物。优化后黄酮发酵培养基中的麦芽糖浓度为46.5 g/L，酵母提取物浓度为6.0 g/L，培养基初始pH值为 4.32，优化后培养基黄酮产量达到(65.3±2.5) mg/L。获得了桑黄黄酮发酵的最佳培养基，为进一步研究桑黄黄酮生物合成调控以及提高桑黄黄酮含量奠定了基础。

Abstract:

-

参考文献/References:

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备注/Memo

备注/Memo:

收稿日期：2019-02-21
基金项目：2015年江苏省“六大人才高峰”资助项目(编号：NY-024)；江苏省科技支撑计划(编号：BE2014338)；江苏省重点研发计划(编号：BE2015361)。
作者简介：谢春芹(1976—)，女，江苏沭阳人，硕士，副教授，主要从事食药用真菌的教学与研究。E-mail：xiechunqin@163.com。
通信作者：任昂，博士，副教授，主要从事食药用真菌遗传与育种研究。E-mail：angren@njau.edu。

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更新日期/Last Update: 2019-07-05