|本期目录/Table of Contents|

[1]张牧原,李军,黄佳,等.南湖菱壳中五没食子酰葡萄糖提取工艺及其抗肿瘤活性研究[J].江苏农业科学,2021,49(5):173-179.
　Zhang Muyuan,et al.Study on extraction technology and anti-tumor activity of pentagalloylglucose from Nanhu water chestnut[J].Jiangsu Agricultural Sciences,2021,49(5):173-179.
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南湖菱壳中五没食子酰葡萄糖提取工艺及其抗肿瘤活性研究(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第5期
页码:
173-179
栏目:
贮藏加工与检测分析
出版日期:
2021-03-05

文章信息/Info

Title:
Study on extraction technology and anti-tumor activity of pentagalloylglucose from Nanhu water chestnut
作者:
张牧原1; 李军2; 黄佳1; 吴霁蓂1; 蒋琦1; 高广春1
1.嘉兴学院医学院，浙江嘉兴 314001； 2.嘉兴职业技术学院现代农业学院，浙江嘉兴 314036
Author(s):
Zhang Muyuan; et al
关键词:
响应面法; 南湖菱壳; 五没食子酰葡萄糖; 抗肿瘤活性
Keywords:
-
分类号:
TS201.1
DOI:
-
文献标志码:
A
摘要:
为明确菱壳中五没食子酰葡萄糖的最佳提取工艺条件，在单因素试验的基础上采用响应面法，以提取率为评价指标研究提取工艺，并采用CCK8法对最优单因素和最佳提取工艺条件下的提取物进行了抗SK-BR-3肿瘤细胞增殖的研究。结果表明，影响提取率的因素依次为提取温度>乙醇浓度>超声时间，乙醇浓度和提取温度的交互作用最强，对提取率的影响最为显著；最佳的提取工艺为料液比1 g∶20 mL，乙醇浓度67%，提取温度80 ℃，超声时间 10 min，预测提取率为0.674 2%，实际提取率为0.675 5%，结果相符。各部分提取物对SK-BR-3乳腺癌细胞增殖具有显著的抑制作用，并且呈现一定的时间和浓度依赖性。在浓度为100 μg/mL时，各部分提取物对受试细胞具有极显著的抑制增殖作用(P<0.01)。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2020-06-05
基金项目：国家自然科学基金青年科学基金(编号：81703667)；国家大学生创新创业训练项目(编号：201810354034)；嘉兴学院大学生创新能力个性化培养项目；浙江省嘉兴市科技计划(编号：2020AY10023)；2020年浙江省新苗人才计划。
作者简介：张牧原(1997—)，男，河南信阳人，从事菱壳资源的综合利用研究。E-mail：849792833@qq.com。
通信作者：高广春，博士研究生，副教授，主要从事天然药物功能性成分的研究。E-mail：gaogcjx@163.com。
更新日期/Last Update: 2021-03-05