|本期目录/Table of Contents|

[1]高华山,陈明辉,齐光,等.金钗石斛总黄酮提取工艺优化及抗氧化活性[J].江苏农业科学,2019,47(24):194-199.
 Gao Huashan,et al.Optimization of extraction process and evaluation of antioxidative activity of total flavonoids from Dendrobium nobile Lindl.[J].Jiangsu Agricultural Sciences,2019,47(24):194-199.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第24期
页码:
194-199
栏目:
贮藏加工与检测分析
出版日期:
2020-02-20

文章信息/Info

Title:
Optimization of extraction process and evaluation of antioxidative activity of total flavonoids from Dendrobium nobile Lindl.
作者:
高华山 陈明辉 齐光 张科 佟伟霜
平顶山学院,河南平顶山 467000
Author(s):
Gao Huashanet al
关键词:
金钗石斛黄酮响应面法抗氧化活性
Keywords:
-
分类号:
R284.2
DOI:
-
文献标志码:
A
摘要:
为了提高金钗石斛中黄酮类化合物的提取得率,在料液比、提取温度、提取时间、乙醇体积分数4个单因素试验基础上,通过二次通用旋转组合设计试验优化金钗石斛总黄酮的超声辅助提取工艺条件,并初步评价其体外抗氧化活性。优化的金钗石斛黄酮提取条件为:提取时间10 min、料液比1 g ∶15 mL,提取温度75 ℃,乙醇体积分数90%。根据修正的最佳工艺条件,进行6次重复试验,测得黄酮提取率的均值为(0.551 5±0.000 4) mg/g,与模型预测值相符。抗氧化试验结果表明,金钗石斛总黄酮对ABTS自由基、DPPH自由基有较好的清除效果,与提取液质量浓度成正比。优化的金钗石斛黄酮提取工艺合理、可行,金钗石斛黄酮具有较强的抗氧化性。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2019-02-21
基金项目:河南省科技攻关项目(编号:172102310211、182102110166、192102310087);高等学校科学技术研究重点项目(编号:18A180026);平顶山学院高层次人才启动基金(编号:PXY-BSQD-2018011、PXY-BSQD-2018010);平顶山学院培育基金(编号:PXY-PYJJ-2019007)。
作者简介:高华山(1983—),男,山东日照人,博士,讲师,主要从事微生物与生化药学研究。E-mail:guoqi
更新日期/Last Update: 2019-12-20