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[1]丁威,邢军,周燕芬,等.二花脸猪卵巢卵泡发育过程中可溶性鸟苷酸环化酶和一氧化氮合酶活性变化及其定位[J].江苏农业科学,2018,46(19):181-186.
　Ding Wei,et al.Bioactivity changes and localization of ovarian nitric oxide synthase (NOS) isoforms and soluble guanylyl cyclase (sGC) α1 and β1 subunits during ovarian follicular development of Erhualian pigs[J].Jiangsu Agricultural Sciences,2018,46(19):181-186.
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二花脸猪卵巢卵泡发育过程中可溶性鸟苷酸环化酶和
一氧化氮合酶活性变化及其定位(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年第19期
页码:
181-186
栏目:
畜牧兽医与水产蚕桑
出版日期:
2018-10-05

文章信息/Info

Title:
Bioactivity changes and localization of ovarian nitric oxide synthase (NOS) isoforms and soluble guanylyl cyclase (sGC) α1 and β1 subunits during ovarian follicular development of Erhualian pigs
作者:
丁威1; 2;  邢军1; 2;  周燕芬3;  刘晨1; 2;  魏全伟2;  石放雄2
1.江苏农林职业技术学院，江苏句容 212400； 2.南京农业大学动物科技学院，江苏南京 210095；3.江苏省农业科学院，江苏南京 210014
Author(s):
Ding Wei; et al
关键词:
猪; 卵巢; 一氧化氮合酶; 可溶性鸟苷酸环化酶; 细胞
Keywords:
-
分类号:
S828.3
DOI:
-
文献标志码:
A
摘要:
为研究在胎猪卵巢发育过程中，NO/sGC/cGMP信号通路对猪卵泡发育的影响，选取70日龄、90日龄胎猪和1日龄仔猪以及120日龄母猪为试验动物，取其卵巢，对其进行免疫组织化学分析，研究一氧化氮合酶［eNOS(endothelial nitric oxide synthase)、iNOS(inducible nitric oxide synthesis)、nNOS(neuronal nitric oxide synthase)］以及可溶性鸟苷酸环化酶(sGCα1、sGCβ1)在卵巢中的表达，并使用化学比色法进行酶活性测定。结果表明，3种NOS在卵巢不同细胞中均有表达，特别是随着卵泡的发育，在膜细胞和颗粒细胞中的表达逐渐增强。而sGCα1和sGCβ1除了在卵巢的腔前卵泡的颗粒细胞中表达，也在黄体细胞和有腔卵泡膜细胞中有较高的表达量。研究发现，sGCα1和sGCβ1在腔前卵泡的颗粒细胞中的表达量最高，且随着卵泡的不断发育，这2种蛋白的表达强度逐渐降低；总NOS、eNOS、iNOS活性在出生前期显著高于出生后期(P<0.05)，nNOS含量在出生前期和出生后期之间差异不显著(P>0.05)。说明NO/sGC/cGMP通路在卵泡的早期发育过程中发挥了重要的作用。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2017-06-01
基金项目：江苏省自然科学基金(编号：BK2011499、BK20171310)；江苏省农业自主创新项目［编号：CX(12)2037］；镇江市科技计划(编号：NY2012027)；江苏农林职业技术学院基金扶持项目(编号：2016kj001)；江苏高校“青蓝工程”中青年学术带头人资助项目(编号：苏教师﹝2017﹞15号)。
作者简介：丁威(1979—)，男，黑龙江巴彦人，博士，副教授，主要从事猪的卵巢卵泡发育调控机制研究。Tel：(0511)87276896
更新日期/Last Update: 2018-10-05