|本期目录/Table of Contents|

[1]邹禹,刘园园,张培江,等.水稻富亮氨酸重复类受体蛋白激酶OsRPK1响应外源生长素的作用研究[J].江苏农业科学,2019,47(08):46-51.
　Zou Yu,et al.Study on role of a rice leucine-rich repeat receptor protein kinase OsRPK1 in response to exogenous auxin[J].Jiangsu Agricultural Sciences,2019,47(08):46-51.
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水稻富亮氨酸重复类受体蛋白激酶OsRPK1响应
外源生长素的作用研究(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第08期
页码:
46-51
栏目:
生物技术
出版日期:
2019-05-19

文章信息/Info

Title:
Study on role of a rice leucine-rich repeat receptor protein kinase OsRPK1 in response to exogenous auxin
作者:
邹禹1;  刘园园2;  张培江1;  钱宝云1;  张炜2
1.安徽省农业科学院水稻研究所，安徽合肥 210031； 2.南京农业大学生命科学学院，江苏南京 210095
Author(s):
Zou Yu; et al
关键词:
水稻富亮氨酸重复类受体蛋白激酶OsRPK1; 原核表达; 外源生长素; 融合蛋白GST-LRRs
Keywords:
-
分类号:
S511.01；Q55
DOI:
-
文献标志码:
A
摘要:
为明确水稻富亮氨酸重复类受体蛋白激酶OsRPK1响应外源生长素的作用机制，首先分析外源生长素2，4-D 对OsRPK1转基因水稻根部表型的影响；其次，异源表达OsRPK1胞外富亮氨酸重复LRRs，检测H3-IAA竞争结合融合蛋白GST-LRRs后的放射性活度以及利用等温滴定量热(ITC)法分析IAA与融合蛋白GST-LRRs相互作用的微热量变化。结果表明，在正常生长条件下，OsRPK1过表达能够抑制水稻侧根的生长；0.01 μmol/L 2，4-D处理 4 d 后发现，OsRPK1抑制表达植株侧根的数量比野生型多112%(P<0.01)，而OsRPK1过表达植株侧根生长受到极显著抑制(P<0.001)；0.1 μmol/L 2，4-D处理10 d后，抑制表达植株不定根的数量相对于野生型多18.2%(P<005)，而过表达植株不定根的生长受到显著抑制(P<0.01)；大肠杆菌BL21(DE3)诱导表达获得了包涵体形式的GST-LRRs融合蛋白，通过复性、纯化获得可溶性融合蛋白；H3-IAA竞争结合融合蛋白GST-LRRs以及IAA溶液滴定融合蛋白的微热量分析都表明OsRPK1与外源生长素未发生直接作用。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2018-12-05
基金项目：国家重点研发计划(编号：2016YFD0101801)；国家自然科学基金(编号：31701409)；安徽省自然科学基金(编号：1408085MKL63)；安徽省农业科学院学科建设项目(编号：17A0101)。
作者简介：邹禹(1986—)，男，安徽安庆人，博士，助理研究员，主要从事水稻基因功能解析及分子育种。Tel：(0551)62160151；E-mail：zouyu0308@126.com。
通信作者：张炜，博士，教授，主要从事植
更新日期/Last Update: 2019-04-20