凡纳滨对虾P53亲水肽段原核表达及抗体制备-《江苏农业科学》

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Title:: Prokaryotic expression and polyclonal antibody preparation of P53 hydrophilic peptides from Litopenaeus vannamei

作者:: 刘欢; 黄明珠; 陈晓燕; 房春娟; 姜雯雯; 朱芳芳; 熊志伟; 江西科技学院护理学院，江西南昌 330098

Author(s):: Liu Huan; et al

关键词:: P53; 原核表达; 纯化; 多克隆抗体

Keywords:: -

分类号:: S917.4

DOI:: -

文献标志码:: A

摘要:: 在哺乳动物细胞中，肿瘤抑制基因P53是目前研究最为广泛和系统的抑癌基因之一。P53参与了DNA损伤修复、细胞周期调控、细胞凋亡及抑制血管生成等过程，在甲壳动物中研究较少。对LvP53的蛋白质全序列进行抗原性分析，将编码亲水肽段DNA连接到Pet32a表达载体上，转化原核表达菌株BL21，利用异丙基-β-D-硫代半乳糖苷(isopropyl β-D-thiogalactoside，简称IPTG)诱导融合蛋白质表达，经蛋白电泳分析表明，融合蛋白主要以包涵体形式存在。利用Ni柱亲和纯化发酵产物，得到较单一的蛋白，以制备多克隆抗体。

Abstract:: -

参考文献/References:

［1］沈文英，阳会军，尹军霞. 南美白对虾的病害及防治研究现状［J］. 水利渔业，2004，24(1)：58-60.
［2］宋盛宪，古群红. 南美白对虾无公害健康养殖技术［J］. 江西水产科技，2006(1)：36-38.
［3］Chanock S J，Benna J，Smith R M，et al. The respiratory burst oxidase［J］. Journal of Biological Chemistry，1994，269(40)：24519-24522.
［4］Muoz M，Cedeo R，Rodríquez J，et al. Measurement of reactive Oxygen intermediate production in haemocytes of the penaeid shrimp，Penaeus vannamei［J］. Aquaculture，2000(191)：89-107.
［5］Valko M，Leibfritz D，Moncol J，et al. Free radicals and antioxidants in normal physiological functions and human disease［J］. The International Journal of Biochemistry & Cell Biology，2007，39(1)：44-84.
［6］Serrano M A, Li Z，Mohan D，et al. DNA-PK，ATM and ATR collaboratively regulate p53-RPA interaction to facilitate homologous recombination DNA repair［J］. Oncogene，2013，32(19)：2452.
［7］Pani G，Koch O R，Galeotti T. The p53-p66shc-manganese superoxide dismutase (MnSOD) network：a mitochondrial intrigue to generate reactive oxygen species［J］. International Journal of Biochemistry & Cell Biology，2009，41(5)：1002-1005.
［8］郭慧，冼健安，王安利. 亚硝酸盐对凡纳滨对虾血细胞毒性及p53基因表达的影响［J］. 水生态学杂志，2015(2)：61-67.
［9］Qian Z Y，Liu T，Liu Q，et al. p53 is involved in shrimp survival via its regulation roles on MnSOD and GPx in response to acute environmental stresses［J］. Comparative Biochemistry and Physiology C-Toxicology & Pharmacology，2014，159(1)：38-51.
［10］侯聪聪. 十足目甲壳动物顶体构架体及精子发生相关蛋白功能研究［D］. 杭州：浙江大学，2015.

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